Product Name: Synaptotagmin-12
Product Number: AB-NN345-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Synaptotagmin-12

Target Alias: SYT-12; SYT12; Synaptotagmin XII; SytXII; Syt XII; Synaptotagmin12; SynaptotagminXII

Product Type Specific: Synaptic protein pan-specific antibody

Antibody Code: NN345-1

Antibody Target Type: Pan-specific

Protein UniProt: Q8IV01

Protein SigNET: Q8IV01

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Fusion protein amino acids 168-255 (Cytoplasmic C2A domain) of mouse Synaptotagmin-12

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: In mouse brain lysates, this antibody detects a ~45 kDa protein by Western blotting.

Antibody Species Reactivity: Mouse | Rat

Antibody Positive Control: 1 µg/ml of SMC-437 was sufficient for detection of Synaptotagmin-12 in 20 µg of transiently overexpressing synaptotagmin-12 COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Antibody Cross Reactivity: Does not cross-react with Synaptotagmin-6 or other Synaptotagmins.

Related Product 1: Synaptophysin pan-specific antibody (Cat. No.: AB-NN370-1)

Related Product 2: Synaptotagmin-3 pan-specific antibody (Cat. No.: AB-NN366-1)

Scientific Background: Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2 (1, 2).