Product Name: TCP1-alpha
Product Number: AB-NN346-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: T-complex protein 1 subunit alpha

Target Alias: p63; Tcp-1; TCP1 alpha; Ccta; TRic; Tp63; 21454; c-cpn; CCT; Tcp1; ccpn; AI528772; Cct1; Tcp 1; c cpn

Product Type Specific: Chaperone protein pan-specific antibody

Antibody Code: NN346-1

Antibody Target Type: Pan-specific

Protein UniProt: P17987

Protein SigNET: P17987

Antibody Type: Monoclonal

Antibody Host Species: Rat

Antibody Ig Isotype Clone: IgG2a

Antibody Immunogen Source: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunoprecipitation | FCM | Immunohistochemistry

Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting. Also detects ~92 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Rabbit | Guinea pig | Hamster | Dog | Pig | S. cerevisiae | C. elegans | Drosophila | Monkey

Antibody Positive Control: 1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Antibody Cross Reactivity: Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.

Related Product 1: TCP1-alpha pan-specific antibody (Cat. No.: AB-NN346-2)

Scientific Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.