Product Name: VAMP1/2
Product Number: AB-NN358-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Vesicle-associated membrane protein 1
Target Alias: SYB1; Synaptobrevin; vesicle associated membrane protein
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN358-1
Antibody Target Type: Pan-specific
Protein UniProt: P23763
Protein SigNET: P23763
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Crude human synaptic immunoprecipitate
Production Method: Protein G purified
Target Alias: SYB1; Synaptobrevin; vesicle associated membrane protein
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN358-1
Antibody Target Type: Pan-specific
Protein UniProt: P23763
Protein SigNET: P23763
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Crude human synaptic immunoprecipitate
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ELISA
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~20-25 kDa proteins. Recognizes VAMP 1 and 2.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ELISA
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~20-25 kDa proteins. Recognizes VAMP 1 and 2.
Antibody Species Reactivity: Human | Rat
Antibody Positive Control: 1 µg/ml of SMC-179 was sufficient for detection of VAMP in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Positive Control: 1 µg/ml of SMC-179 was sufficient for detection of VAMP in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background: VAMP-1, also known as vesicle-associated membrane protein, is an 18kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane (1). Synaptobrevins, Syntakins and the 25kDa synaptosomal associated SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (2). VAMP-1 is also one of the key proteins in the SNARE complex which is involved in regulated exocytosis (3).
References
[1] Trimble W.S., Cowan D.M., and Scheller R.H. (1988) Proc Natl Acad Sci USA. 85: 4538-4542.
[2] Nabokina S.M. (2001) Bulletin of Experimental Bio and Med. 131(2): 130-131.
[3] Berglund L., Hoffman H.J., Dahl R., and Peterson T.E. (1999) Biochem Biophys Research Communicaitons. 264(3): 777-780.
[1] Trimble W.S., Cowan D.M., and Scheller R.H. (1988) Proc Natl Acad Sci USA. 85: 4538-4542.
[2] Nabokina S.M. (2001) Bulletin of Experimental Bio and Med. 131(2): 130-131.
[3] Berglund L., Hoffman H.J., Dahl R., and Peterson T.E. (1999) Biochem Biophys Research Communicaitons. 264(3): 777-780.
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