Product Name: Versican
Product Number: AB-NN360-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Versican core protein
Target Alias: Chondroitin sulfate proteoglycan 2; CSPG2; ERVR; GHAP; PG-M; VCAN; Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Glial hyaluronate binding protein; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; Large fibroblast proteoglycan; PGM; V1 Neo; Versican proteoglycan; Versican V0; WGN 1; WGN; WGN1
Product Type Specific: Versican pan-specific antibody
Antibody Code: NN360-1
Antibody Target Type: Pan-specific
Protein UniProt: P13611
Protein SigNET: P13611
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Target Alias: Chondroitin sulfate proteoglycan 2; CSPG2; ERVR; GHAP; PG-M; VCAN; Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Glial hyaluronate binding protein; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; Large fibroblast proteoglycan; PGM; V1 Neo; Versican proteoglycan; Versican V0; WGN 1; WGN; WGN1
Product Type Specific: Versican pan-specific antibody
Antibody Code: NN360-1
Antibody Target Type: Pan-specific
Protein UniProt: P13611
Protein SigNET: P13611
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Immunogen Source: Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a >350 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-439 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Potency: In mouse brain lysates, this antibody detects a >350 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-439 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels.
References
[1] Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998.
[2] Evanko S.P., Angello J.C. and Wight T.N. (1999) Arterioscler. Thromb. Vasc. Biol. 4:1004-1013.
[3] Lemire J.M. et al. (1999) Arterioscler. Thromb. Vasc. Biol. 7: 1630-1639.
[4] Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623.
[5] Touab M., Villena J., Barranco C., Arumi-Uria M. and Bassols A. (2002) Am. J. Pathol. 2: 549-557.
[1] Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998.
[2] Evanko S.P., Angello J.C. and Wight T.N. (1999) Arterioscler. Thromb. Vasc. Biol. 4:1004-1013.
[3] Lemire J.M. et al. (1999) Arterioscler. Thromb. Vasc. Biol. 7: 1630-1639.
[4] Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623.
[5] Touab M., Villena J., Barranco C., Arumi-Uria M. and Bassols A. (2002) Am. J. Pathol. 2: 549-557.
© Kinexus Bioinformatics Corporation 2017