Product Name: SQSTM1
Product Number: AB-NN375-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Sequestosome-1
Target Alias: EBI3-associated protein of 60 kDa; ORCA; Osi; SQSTM 1; ZIP 3; A170; SQSTM1; Ubiquitin binding protein p62; Protein kinase C-zeta-interacting protein; FTDALS3; Ubiquitin-binding protein p62; PKC-zeta-interacting protein; EBIAP; Sqstm1; Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa; EBI3 associated protein p60; EBI3 associated protein of 60 kDa; SQSTM_HUMAN; OSF-6; Paget disease of bone 3; p62B; p60; PDB 3; EBI 3 associated protein p60; MGC127197; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; EBI 3 associated protein of 60 kDa; ZIP3; STAP; p62; Sequestosome 1; OSIL; STONE14; Phosphotyrosine independent ligand for the Lck SH2 domain p62; PDB3; Oxidative stress induced like; ZIP
Product Type Specific: Adapter/scaffold protein pan-specific antibody
Antibody Code: NN375-1
Target Alias: EBI3-associated protein of 60 kDa; ORCA; Osi; SQSTM 1; ZIP 3; A170; SQSTM1; Ubiquitin binding protein p62; Protein kinase C-zeta-interacting protein; FTDALS3; Ubiquitin-binding protein p62; PKC-zeta-interacting protein; EBIAP; Sqstm1; Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa; EBI3 associated protein p60; EBI3 associated protein of 60 kDa; SQSTM_HUMAN; OSF-6; Paget disease of bone 3; p62B; p60; PDB 3; EBI 3 associated protein p60; MGC127197; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; EBI 3 associated protein of 60 kDa; ZIP3; STAP; p62; Sequestosome 1; OSIL; STONE14; Phosphotyrosine independent ligand for the Lck SH2 domain p62; PDB3; Oxidative stress induced like; ZIP
Product Type Specific: Adapter/scaffold protein pan-specific antibody
Antibody Code: NN375-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13501
Protein SigNET: Q13501
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the C-terminal of Human SQSTM1 (AA 335-345)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Protein UniProt: Q13501
Protein SigNET: Q13501
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the C-terminal of Human SQSTM1 (AA 335-345)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects weak-medium ~62 and 40 kDa isoform bands in mouse brain lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: A 1:1000 dilution of SPC-637 was sufficient for detection of SQSTM1 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects weak-medium ~62 and 40 kDa isoform bands in mouse brain lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: A 1:1000 dilution of SPC-637 was sufficient for detection of SQSTM1 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and Jurkat cell lysates.
Scientific Background: SQSTM1 or Sequestome 1 (p62) is a ubiquitin binding protein that interacts with ubiqutin, providing a scaffold for several signaling proteins and triggering degradation of proteins throught the proteasome or lysossome. This gene regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in this gene result in sporadic and familial Paget disease of bone. SQSTM1 also binds autophagosomal membrane protein LC3/ATG8.
References
[1] Kirkin V. et al. (2009) Mol Cell. 34: 259-269.
[2] Seibenhener M.L. et al. (2007) FEBS Lett. 581: 175-179.
[3] Komatsu M. et al. (2010) Nat Cell Biol. 12: 213-223.
[4] Bjørkøy G. et al. (2006) Autophagy. 2: 138-139.
[5] Pankiv S. et al. (2007) J Biol Chem. 282: 24131-24145.
[1] Kirkin V. et al. (2009) Mol Cell. 34: 259-269.
[2] Seibenhener M.L. et al. (2007) FEBS Lett. 581: 175-179.
[3] Komatsu M. et al. (2010) Nat Cell Biol. 12: 213-223.
[4] Bjørkøy G. et al. (2006) Autophagy. 2: 138-139.
[5] Pankiv S. et al. (2007) J Biol Chem. 282: 24131-24145.
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