Target Full Name: Tetratricopeptide repeat domain 10

Target Alias: D13S1056E; TPR repeat protein 10; Ift88; Recessive polycystic kidney disease protein Tg737 homologue; IFT88_HUMAN; Polaris homologue; Probe hTg737 (polycystic kidney disease; autosomal recessive); TG737; TTC10; DAF19; IFT88; hTg737; Intraflagellar transport 88 homologue; MGC26259; Tetratricopeptide repeat protein 10; Intraflagellar transport protein 88 homologue; RP11-172H24.2

Product Type Specific: IFT88 pan-specific antibody

Antibody Code: NN377-1

Antibody Target Type: Pan-specific

Protein UniProt: Q13099

Protein SigNET: Q13099

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Synthetic peptide from the mid to C-terminal of Human IFT88 (AA 674-686)

Production Method: Peptide Affinity purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a medium ~94 kDa protein band in A549 cells by Western blotting.

Antibody Species Reactivity: Human | Mouse

Antibody Positive Control: A 1:1000 dilution of SPC-679 was sufficient for detection of IFT88 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and A549 cell lysates.