Product Name: VEGFR2-pY1214
Product Number: AB-PK133
Size: 25 µg      Price:89.00
      $US
Target Full Name: Receptor protein-tyrosine kinase vascular endothelial growth factor receptor 2 Tyr-121 phosphosite

Target Alias: A type III receptor tyrosine kinase; CD309; EC 2.7.10.1; FLK1; Kinase insert domain receptor; Protein-tyrosine kinase receptor Flk-1; Vascular endothelial growth factor receptor 2; VEGFR; VEGFR2; VGFR2; VGR2

Product Type Specific: Phosphosite-specific polyclonal antibody for human VEGFR2 Y1214 phosphosite

Antibody Code: PK133

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y1214

Protein UniProt: P35968

Protein SigNET: P35968

Antibody Type: Polyclonal
Antibody Host Species: Rabbit

Antibody Ig Isotype Clone: IgG

Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human VEGFR2 Tyr-1214 phosphosite (Phe-His-pTyr-Asp-Asn)

Antibody Immunogen Sequence: FHpYDN

Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human VEGFR2 Y1214 phosphosite

Production Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol

Storage Conditions: -20°C

Storage Stability: > 1 year

Product Use: Western blotting, Immunohistochemistry (IHC)

Antibody Dilution Recommended: 2 µg/mL

Antibody Potency: Very strong

Antibody Species Reactivity: Human, Mouse

Antibody Positive Control: Y1214 phosphorylated VEGFR2

Antibody Specificity: Very high

Antibody Cross Reactivity: No strong cross-reactivities with other proteins.

Related Product 1: VEGFR2-1 pan-specific antibody (Cat. No.: AB-NK245-1)

Related Product 2: VEGFR2-2 pan-specific antibody (Cat. No.: AB-NK245-2)

Related Product 3: VEGFR2-3 pan-specific antibody (Cat. No.: AB-NK245-3)

Related Product 4: VEGFR2-pY1054 phosphosite-specific antibody (Cat. No.: AB-PK852)

Scientific Background: VEGFR2 a receptor-tyrosine kinase of the VEGFR family that has high affinity for VEGF and VEGF-C as ligands. Engagement of these ligands induces autophosphorylation, catalytic activation, and recruitment of proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. VEGFR2 plays a key role in vascular development and regulation of vascular permeability. Interaction with the HIV-1 Tat protein enhances angiogenesis in Kaprosi's sarcoma lesions. Defects in VEGFR2 are associated with susceptibility to hemangioma capillary infantile (HCI), which are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium responsible for about 10% of infant tumours.
Figure 1. Immunoblotting of various cell lines with AB-PK133 antibody at a 500 X dilution. The target protein VEGFR2 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 31115.


Figure 2. Immunoblotting of various cell lines with AB-PK133 antibody at a 500 X dilution. The target protein VEGFR2 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 24341.


References

[1] Takahashi T, Yamaguchi S, Chida K, Shibuya M. A single autophosphorylation site on KDR/Flk-1 is essential for VEGF-A-dependent activation of PLC-gamma and DNA synthesis in vascular endothelial cells. EMBO J. 2001 Jun 1;20(11):2768-78. PMID: 11387210.

[2] Lamalice L, Houle F, Jourdan G, Huot J. Phosphorylation of tyrosine 1214 on VEGFR2 is required for VEGF-induced activation of Cdc42 upstream of SAPK2/p38. Oncogene. 2004 Jan 15;23(2):434-45. PMID: 14724572.