Target Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2

Target Alias: AAPK2; AMPK alpha-2 chain; Kinase AMPK-alpha2; AMPK2; PRKAA; PRKAA2 ENSG00000162409

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK522

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S377

Protein UniProt: P54646

Protein SigNET: P54646

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human AMPKa2 (PRKAA2) sequence peptide Cat. No.: PE-04AHL80

Antibody Immunogen Sequence: IAD(pS)PKARC

Antibody Immunogen Description: Corresponds to amino acid residues I374 to C382; In the C-terminal half of the protein after the kinase catalytic domain. This is the major in vivo phosphorylation sites in AMPKa2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.