Target Full Name: Ankyrin repeat domain protein-serine kinase 3 (RIPK4, DIK)

Target Alias: ANKK2; Ankyrin repeat domain protein 3; DIK; Kinase ANKRD3; PKC-delta-interacting protein kinase; PKC-regulated kinase PKK; Receptor-interacting serine-threonine kinase 4; RIP4; RIPK4; DIK

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK523

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S438

Protein UniProt: P57078

Protein SigNET: P57078

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human ANKRD3 sequence peptide Cat. No.: PE-04AIH99

Antibody Immunogen Sequence: LSL(pS)FER(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues L435 to R441; In the region between the kinase catalytic and Ank_2 domains.The major site of phosphorylation in ANKRD3.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.