Product Name: Blk-pY188
Product Number: AB-PK542
Size: 25 µg      Price:89.00
      $US
Target Full Name: B lymphoid tyrosine kinase

Target Alias: P55-Blk

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK542

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y188

Protein UniProt: P51451

Protein SigNET: P51451

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Blk sequence peptide Cat. No.: PE-04AIR99

Antibody Immunogen Sequence: GGY(pY)ISP(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G185 to P191; In the SH2 domain. One of the two major in vivo phosphorylation sites in Blk.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in A431, Jurkat and T98G cells and sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, Jurkat and T98G cells and sea star oocytes.

Related Product 1: Blk-pY188 blocking peptide

Related Product 2: Blk-pY187 phosphosite-specific antibody (Cat. No.: AB-PK541)

Related Product 3: Blk-pY389 phosphosite-specific antibody (Cat. No.: AB-PK543)

Scientific Background: Blk is a protein-tyrosine kinase that is a member of the TK group of protein kinases in the Src family. It has been reported that Blk is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells. However, this kinase has low to reasonably high expression (e.g. skeletal muscle, kidney, thyroid, breast) in most tested human tissues except in the brain and spinal cord where it is poorly expressed. Orthologues of Blk are highly conserved in mammals and birds. Blk is inhibited by phosphorylation at Y501. Blk has been linked with the development of colorectal adenocarcinomas. Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk.
Figure 1. Epitope mapping of BLK-pY188 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to BLK-pY188.