Product Name: CaMK1a-pT177
Product Number: AB-PK553
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 1 alpha
Target Alias: Calcium/calmodulin-dependent protein kinase I; Calcium/calmodulin-dependent protein kinase type I; CAM kinase I; CAMK1; CaMK1-alpha; CaMKI; KCC1; KCC1A; Kinase CaMK1-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK553
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T177
Protein UniProt: Q14012
Protein SigNET: Q14012
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: Calcium/calmodulin-dependent protein kinase I; Calcium/calmodulin-dependent protein kinase type I; CAM kinase I; CAMK1; CaMK1-alpha; CaMKI; KCC1; KCC1A; Kinase CaMK1-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK553
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T177
Protein UniProt: Q14012
Protein SigNET: Q14012
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK1a (CaMKI) sequence peptide Cat. No.: PE-04AIZ95
Antibody Immunogen Sequence: VLS(pT)ACG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V174 to G180; In the protein kinase catalytic domain.
Antibody Immunogen Sequence: VLS(pT)ACG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V174 to G180; In the protein kinase catalytic domain.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No cross-reactivities, but background is high in A431 cells. In HEK-293 has a slightly higher background with FBS treatment, but not with phenylarsine oxide (PAO) treatment.
Related Product 1: CaMK1a-pT177 blocking peptide
Related Product 2: Autocamtide-2 peptide - Autocamtide 2 CaMK2-derived peptide substrate
Related Product 3: CAMK1Subtide - CaMK1a protein kinase substrate peptide
Related Product 4: ZIPtide KinSub - DAPK3 (ZIPK) protein kinase substrate peptide
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No cross-reactivities, but background is high in A431 cells. In HEK-293 has a slightly higher background with FBS treatment, but not with phenylarsine oxide (PAO) treatment.
Related Product 1: CaMK1a-pT177 blocking peptide
Related Product 2: Autocamtide-2 peptide - Autocamtide 2 CaMK2-derived peptide substrate
Related Product 3: CAMK1Subtide - CaMK1a protein kinase substrate peptide
Related Product 4: ZIPtide KinSub - DAPK3 (ZIPK) protein kinase substrate peptide
Scientific Background: CaMK1a is a protein-serine/threonine kinase of the CAMK group and CAMK1 family. Phosphorylation at Thr-177 increases its phosphotransferase activity by approximately 25-fold in a Ca(2+)-CaM dependent manner. Thr-177 is phosphorylated by CAMKK1 or CAMKK2. CaMK1a and CaMKK1 regulate the G(O)-G(1) restriction check point for cell cycle transition in human breast cancer cells. Target substrates for CaMK1a includes CREB1, ATF1, CFTR, MYL9, and SYN1/synapsin I.
Figure 1. Dot blotting CaMK1a-pT177 antibody with recombinant purified proteins.
Figure 2. Identification of phosphosites related to CaMK1a-pT177.
© Kinexus Bioinformatics Corporation 2017