Product Name: CaMK1d-pT180
Product Number: AB-PK554
Size: 25 µg      Price:89.00
      $US
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 1 delta

Target Alias: CaMK1D; CaMK1-delta; CaM-KI delta; EC 2.7.11.17; KCC1D

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK554

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T180

Protein UniProt: Q8IU85

Protein SigNET: Q8IU85

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CaMK1d sequence peptide Cat. No.: PE-04AJA99

Antibody Immunogen Sequence: VMS(pT)ACG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues V177 to G183; In the protein kinase catalytic domain.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-50 kDa.

Antibody Specificity: High

Scientific Background: CaMK1d is a protein-serine/threonine kinase of the CaMK group and CaMK1 family. It is activated by Ca2+/calmodulin. The binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CaMKK1 and CaMKK2. At least in part, it can also be activated by CaMKK1 in a calcium-independent manner. It is associated with calcium-triggered CaMKK-CaMK1 signalling cascade and activates CREB- and CREM-BETA-dependent gene transcription. A K52A mutation leads to a loss of phosphotransferase function.
Figure 1. Dot blotting CaMK1d-pT180 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of CaMK1d-pT180 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to CaMK1d-pT180.