Product Name: CDK5-pY15
Product Number: AB-PK570
Size: 25 µg      Price:89.00
      $US
Target Full Name: Cyclin-dependent protein-serine kinase 5

Target Alias: Cell division protein kinase 5; CRK6; PSSALRE; TAU protein kinase II; TPKII; ENSG00000164885

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK570

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y15

Protein UniProt: Q00535

Protein SigNET: Q00535

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CDK5 sequence peptide Cat. No.: PE-04AJO95
Antibody Immunogen Sequence: EGT(pY)GTV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E12 to V18; In the protein kinase catalytic domain in subdomain I.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 29-35 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells and sea star oocytes.

Related Product 1: CDK5-pY15 blocking peptide

Related Product 2: CDK5Selectide - CDK5 protein kinase substrate peptide

Scientific Background: CDK5 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is required for cell cycle arrest in neuronal cells. It is activated by binding p35 protein. Phosphorylation at S159 increases phosphotransferase activity. Phosphorylation at T14 and Y15 inhibits phosphotransferase activity. CDK5 has been observed to be proline-directed and to phosphorylate SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. CDK5 has been implicated in Aneurysmal bone cyst, which is a bone cancer that will affect bone, endothelial tissue, and bone marrow.
Figure 1. Dot blotting CDK5-pY15 antibody with recombinant purified proteins.


Figure 2. Identification of phosphosites related to CDK5-pY15.