Product Name: CDK6-pY13
Product Number: AB-PK571
Size: 25 µg      Price:89.00
      $US
Target Full Name: Cyclin-dependent protein-serine kinase 6

Target Alias: CRK2; Cyclin-dependent kinase 6; Kinase CDK6; PLSTIRE; Serine/threonine protein kinase PLSTIRE; MGC59692; CCDS5628.1; ENSG00000105810

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK571

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y13

Protein UniProt: Q00534

Protein SigNET: Q00534

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CDK6 sequence peptide Cat. No.: PE-04AJP95

Antibody Immunogen Sequence: DQQ(pY)ECV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues D10 to V16; In the protein kinase catalytic domain in subdomain I.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-38 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactivities detected in MCF7, and also in HeLa cells, except phenylarsine oxide (PAO) increases 27 kDa protein immunoreactivity in HeLa cells. In sea star oocytes, this antibody cross-reacted with phospho-CDK1 and phospho-ERK1.

Related Product 1: CDK6-pY13 blocking peptide

Related Product 2: CDK6-pY24 phosphosite-specific antibody (Cat. No.: AB-PK572)

Related Product 3: CDK4/6Selectide - CDK4 protein kinase substrate peptide

Scientific Background: CDK6 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is involved in cell cycle regulation and differentiation, which promotes G1/S transition. Phosphorylation at T177 increases phosphotransferase activity. Germline mutations in CDK6 are hypothesized to lead to a predisposition to melanoma. A197T mutation is found in family with autosomal recessive primary microcephaly-12 (MCPH12).
Figure 1. Dot blotting CDK6-pY13 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of CDK6-pY13 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to CDK6-pY13.