Product Name: CDKL5-pY171
Product Number: AB-PK576
Size: 25 µg      Price:89.00
      $US
Target Full Name: Cyclin-dependent kinase-like 5; Serine-threonine-protein kinase 9

Target Alias: Cyclin-dependent kinase-like 5; Kinase CDKL5; STK9

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK576

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y171

Protein UniProt: O76039

Protein SigNET: O76039

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CDKL5 (STK9) sequence peptide Cat. No.: PE-04ACN90

Antibody Immunogen Sequence: YTE(pY)VAT(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues Y168 to T174; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-120 kDa.

Related Product 1: CDKL5-pY171 blocking peptide

Scientific Background: CDKL5 is a protein-serine/threonine kinase of the CMGC group and CDKL family. It is likely that phosphorylation at Y24 is inhibitory and phosphorylation at Y171 is required for catalytic activity based on homology with other CDK's. It has been reported to phosphorylate MECP2. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. It has also been linked with Rett Syndrome, which is a neuro-development disorder affecting psychomotor development, autistic like features, seizures, and slow head growth. Rett Syndrome is also associated with neuroblastomas.
Figure 1. Epitope mapping of CDKL5-pY171 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to CDKL5-pY171.