Product Name: Chk2-pT68
Product Number: AB-PK581
Size: 25 µg      Price:89.00
      $US
Target Full Name: Checkpoint protein-serine kinase 2

Target Alias: Cds1; CHEK2; Chk2 checkpoint homologue (S. pombe); HuCds1; LFS2; PP1425; RAD53; RP11-436C9.1; bA444G7; CCDS13843.1; ENSG00000183765

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK581

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T68

Protein UniProt: O96017

Protein SigNET: O96017

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Chk2 (CHEK2) sequence peptide Cat. No.: PE-04AJY99

Antibody Immunogen Sequence: TVS(pT)QEL(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues T65 to L71; In the N-terminal portion of the kinase before the FHA domain.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-68 kDa.
Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells and sea star oocytes.

Related Product 1: Chk2-pT68 blocking peptide

Related Product 2: Chk2-pT383 phosphosite-specific antibody (Cat. No.: AB-PK580)

Related Product 4: Chk2Selectide - Chk2 (CHEK2) protein kinase substrate peptide

Scientific Background: Chk2 (CHEK2) is a protein-serine/threonine kinase of the CAMK group and RAD53 family. It is required for cell cycle arrest, activation of DNA repair, and the promotion of apoptosis in response to double-stranded breaks in DNA. Chk2 is activated through phosphorylation by ATM and ATR at T68. It is activated by phosphorylation at T68 by MLTK in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner. It is also activated by phosphorylation at S19, T26, S28, S33, S35, T383, T387 and S516. The effects of Chk2 are mediated through the phosphorylation and inhibition of various downstream effectors, including CDC25A, CDC25B, and CDC25C. In addition, Chk2 phosphorylates p53 and BRCA to mediate cell cycle arrest in response to DNA damage. This kinase shows high variability in human tissue distribution with the highest levels detected in spleen and tonsils, and is notably absent in brain and spinal cord. Orthologues of Chk2 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on S20 and p53 stabilization. Chk2 has been linked with the development of Li-Fraumeni Syndrome 2 (LFS2), breast, colon, and prostate cancers, and osteosarcomas.
Figure 1. Dot blotting Chk2-pT68 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of Chk2-pT68 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to Chk2-pT68.