Target Full Name: Casein protein-serine kinase 2 alpha/ alpha prime

Target Alias: Casein kinase 2, alpha 1 polypeptide; Casein kinase II, alpha chain; CK II; CK2-alpha1; CKIIA; CSK21; CSNK2A1; Kinase CK2-alpha1;

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK582

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y255

Protein UniProt: P68400

Protein SigNET: P68400

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CK2a1 (CSNK2A1) sequence peptide Cat. No.: PE-04AJZ95

Antibody Immunogen Sequence: EDL(pY)DYI(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E252 to I258; In the last quarter of the kinase catalytic domain. This is the major in vivo phosphorylation site in CK2a.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.