Product Name: EIF2AK3-pT982
Product Number: AB-PK604
Size: 25 µg      Price:89.00
      $US
Target Full Name: Eukaryotic translation initiation factor 2-alpha kinase 3

Target Alias: DKFZp781H1925; E2AK3; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; HRI; Kinase PEK; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK604

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T982

Protein UniProt: Q9NZJ5

Protein SigNET: Q9NZJ5

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human EIF2AK3 (PERK) sequence peptide Cat. No.: PE-04AON99

Antibody Immunogen Sequence: ARH(pT)GQV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues A889 to V895; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.

Antibody Specificity: Medium

Antibody Cross Reactivity: ~50 kDa cross-reactive protein in MCF7 cells and an ~80 kDa protein in HeLa cells.

Related Product 1: EIF2AK3-pT982 blocking peptide

Scientific Background: PERK (EIF2AK3) is a protein-serine/threonine kinase of the Other group and PEK family. It serves as a critical effector of unfolded protein response-induced G1 growth arrest. Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and induction of its phosphotransferase activity.
Figure 1. Epitope mapping of EIF2AK3-pT982 antibody with similar phosphopeptides on dot blots.


Figure 2. Epitope mapping of EIF2AK3-pT982 antibody with similar phosphopeptides on dot blots.