Product Name: GSK3a-pS278+pY279
Product Number: AB-PK647
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: Glycogen synthase-serine kinase 3 alpha
Target Alias: DKFZp686D0638; Factor A; Glycogen synthase kinase 3 alpha; GSK-3 alpha; GSK3-alpha; Kinase GSK3-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK647
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S278+Y279
Protein UniProt: P49840 Protein SigNET: P49840 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GSK3a sequence peptide Cat. No.: PE-04ACY95
Antibody Immunogen Sequence: NV(pS)(pY)ICS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N276 to S282; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-54 kDa.
Scientific Background: GSK3a is a protein-serine/threonine kinase of the CMGC group and GSK family. It is highly active following tyrosine phosphorylation within its activation T loop. Phosphorylation at Y279 increases phosphotransferase activity and promotes nuclear localization. Phosphorylation at S21 inhibits phosphotransferase activity. Through direct phosphorylation, it can inactivate glycogen synthase (for insulin regulation), CTNNB1/beta-catenin, APC, and AXIN1. GSK3 has been proposed as a tumour suppressor protein (TSP), since with loss of GSK3 there is an increase in WNT signalling, with a beta-catenin stabilization, leading to tumourigenesis. GSK3 has also been proposed to act as an oncoprotein (OP), as acute leukemia required GSK3 for cell proliferation. In most human cancers, it is up-regulated in expression about 1.6-times the rate observed for most protein kinases.
Figure 1. Epitope mapping of GSK3a-pS278+pY279 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to GSK3a-pS278+pY279.