Product Name: GSK3a-pS278+pY279
Product Number: AB-PK647
Size: 25 µg      Price:89.00
      $US
Target Full Name: Glycogen synthase-serine kinase 3 alpha

Target Alias: DKFZp686D0638; Factor A; Glycogen synthase kinase 3 alpha; GSK-3 alpha; GSK3-alpha; Kinase GSK3-alpha

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK647

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S278+Y279

Protein UniProt: P49840

Protein SigNET: P49840

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human GSK3a sequence peptide Cat. No.: PE-04ACY95
Antibody Immunogen Sequence: NV(pS)(pY)ICS(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues N276 to S282; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-54 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, or in sea star oocytes.

Related Product 1: GSK3a-pS278+pY279 blocking peptide

Related Product 2: GSK3a-BKCD pan-specific antibody (Cat. No.: AB-NK069-3)

Related Product 3: GSK3a-pT19+pS21 phosphosite-specific antibody (Cat. No.: AB-PK648)

Related Product 4: GSK3a-pY279 phosphosite-specific antibody (Cat. No.: AB-PK649)

Related Product 5: GSK3a-pY284+Y285 phosphosite-specific antibody (Cat. No.: AB-PK650)

Related Product 6: GSK3Selectide - GSK3 protein kinase substrate peptide
Scientific Background: GSK3a is a protein-serine/threonine kinase of the CMGC group and GSK family. It is highly active following tyrosine phosphorylation within its activation T loop. Phosphorylation at Y279 increases phosphotransferase activity and promotes nuclear localization. Phosphorylation at S21 inhibits phosphotransferase activity. Through direct phosphorylation, it can inactivate glycogen synthase (for insulin regulation), CTNNB1/beta-catenin, APC, and AXIN1. GSK3 has been proposed as a tumour suppressor protein (TSP), since with loss of GSK3 there is an increase in WNT signalling, with a beta-catenin stabilization, leading to tumourigenesis. GSK3 has also been proposed to act as an oncoprotein (OP), as acute leukemia required GSK3 for cell proliferation. In most human cancers, it is up-regulated in expression about 1.6-times the rate observed for most protein kinases.
Figure 1. Epitope mapping of GSK3a-pS278+pY279 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to GSK3a-pS278+pY279.