Product Name: GUK1-pY53
Product Number: AB-PK652
Size: 25 µg      Price:89.00
      $US
Target Full Name: Guanylate kinase

Target Alias: GMK; GMP kinase; Guanylate kinase 1; KGUA

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK652

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y53

Protein UniProt: Q16774

Protein SigNET: Q16774

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human GUK1 sequence peptide Cat. No.: PE-04AMT99

Antibody Immunogen Sequence: GKD(pY)YFV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G50 to V56; In the first third of the Guanylate_kin domain. Is the major in vivo site of phosphorylation of GUK1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in MCF7 cells and sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 23-28 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: Strong ~220, 50 and 35 kDa immuno-reactive proteins wre detected in sea star oocytes; a ~47 kDa cross-reactive proteins in HepG2 and MCF7 cells; and a strong 40 kDa cross-reactive protein in MCF7 cells.

Related Product 1: GUK1-pY53 blocking peptide

Scientific Background: GUK1 is a protein-serine/threonine kinase of the Atypical group and family. It is essential for recycling GMP and indirectly, cGMP.
Figure 1. Epitope mapping of GUK1-pY53 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to GUK1-pY53.