Product Name: Lyn-pY508

Product Number: AB-PK688

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Yes-related protein-tyrosine kinase; Tyrosine-protein kinase Lyn

Target Alias: JTK8; Kinase Lyn; KPM; Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene; FLJ26625; CCDS6162.1; ENSG00000147507

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK688

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y508

Protein UniProt: P07948

Protein SigNET: P07948

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Lyn sequence peptide Cat. No.: PE-04ANN90

Antibody Immunogen Sequence: EGQ(pY)QQQ(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E505 to Q511; Near the C-terminus of the kinase

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.

Scientific Background: Lyn is a protein-tyrosine kinase of the TK group and Src family. It is important in signal transduction from cell surface receptors and in the regulation of innate and adaptive immune responses and other cellular functions. Phosphorylation of T503 inhibits its phosphotransferase activity.This kinase is highly expressed and widely distributed in most tested human tissues. Lyn deficiency is involved in defects in some mast cell functions. It interacts with phosphorylated LIME1 and with CD79A upon BCR activation. Lyn is inhibited by phosphorylation at T503. It interacts with a variety of cell surface receptors on the cytoplasmic side of the plasma membrane. Lyn is biosynthetically transported to the plasma membrane via the Golgi pool of caveolin along the secretory pathway that requires its catalytic domain, but not activity. Lyn is expressed preferentially in B cells and can be bound in the plasma membrane with IgM, where it participates in antigen-mediated signal transduction. Crosslinking of membrane-bound IgM with antibody induces rapid increase in activities of Lyn and Lyn-associated PI 3-kinase. Lyn may be involved in the changes that are related to tumour pathogenesis. It is constitutively phosphorylated and activated in cells of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) patients. Abnormally enhanced expression levels of activation of Lyn signalling can be critical in survival and proliferation of some types of cancers, inclduing breast carcinomas.

Figure 1. Epitope mapping of Lyn-pY508 antibody with similar phosphopeptides on dot blots.

Figure 2. Identification of phosphosites related to Lyn-pY508.