Product Name: MERTK-pY753
Product Number: AB-PK704
Size: 25 µg      Price:89.00
      $US
Target Full Name: Tyrosine-protein kinase Mer; Proto-oncogene tyrosine-protein kinase MER

Target Alias: C-mer; C-mer proto-oncogene tyrosine kinase; Kinase Mer; MERK; MERTK; MGC133349Proto-oncogene tyrosine-protein kinase MER precursor; Receptor tyrosine kinase MerTK; RP38; MGC133349; CCDS2094.1; ENSG00000153208

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK704

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y753

Protein UniProt: Q12866

Protein SigNET: Q12866

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MERTK (MER) sequence peptide Cat. No.: PE-04ANZ95

Antibody Immunogen Sequence: SGD(pY)YRQG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues S750 to G757; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-115 kDa.

Related Product 1: MERTK-pY753 blocking peptide

Scientific Background: MERTK (MER) is a protein-tyrosine kinase of the TK group and Axl family. It is a receptor kinase that functions in the regulation of cell survival, angiogenesis, migration and invasion, differentiation, and phagocytosis of apoptotic cells. Phosphorylation of Y749, Y753 and Y754 increases phosphotransferase activity.
Figure 1. Epitope mapping of MERTK-pY753 antibody with similar phosphopeptides on dot blots.


Figure 2. Epitope mapping of MERTK-pY753 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to MERTK-pY753.