Product Name: Met-pY1234+pY1235
Product Number: AB-PK711
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: Hepatocyte growth factor (HGF) receptor-tyrosine kinase
Target Alias: C-met; Hepatocyte growth factor receptor; HGF receptor; HGFR; HGF-SF receptor; Kinase Met; Met proto- oncogene tyrosine kinase; Met proto-oncogene; RCCP2; ENSG00000105976
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK711
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1234+Y1235
Protein UniProt: P08581 Protein SigNET: P08581 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Met sequence peptide Cat. No.: PE-04AFO99
Antibody Immunogen Sequence: KE(pY)(pY)SVH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K1232 to H1238; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-155 kDa.
Related Product 1: Met-pY1234+pY1235 blocking peptide
Scientific Background: Met (HGFR) is a protein-tyrosine kinase of the TK group and Met family. It is activated by binding hepatocyte growth factor (HGF), which induces dimerization and autophosphorylation. Phosphorylation of Y1003 increases phosphotransferase activity and induces interaction with Cbl. Phosphorylation of Y1230 and Y1235 increases phosphotransferase activity. Phosphorylation of Y1234 increases phosphotransferase activity and induces interaction with Grb2. Phosphorylation of S985 inhibits phosphotransferase activity. Phosphorylation of Y1349 inhibits phosphotransferase activity, but induces interaction with FAK, Gab1, Grb2, Met, PIK3R1, PLCG1 and Src. Met mediates scattering, proliferation, survival, and morphogenesis of cells. Some immediate regulators that bind to Met after activation with HGF include PLCG1, PIK3R1, GRB2, SRC, STAT3 or the adapter GAB1. Met is a known oncoprotein (OP). Cancer-related mutations in human tumours point to a gain of function of the protein kinase. The active form of the protein kinase normally acts to promote tumour cell proliferation. Hepatocellular carcinoma (HCC) occurs with higher risk in those that have chronic HCV (hepatitis C virus) or HBV (hepatitis B virus) infection, or aflatoxin exposure in diet, or alcohol-induced cirrhosis, or another form of cirrhosis.
Figure 1. Epitope mapping of Met-pY1234+pY1235 antibody with similar phosphopeptides on dot blots.
Figure 2. Epitope mapping of Met-pY1234+pY1235 antibody with similar phosphopeptides on dot blots.
Figure 3. Identification of phosphosites related to Met-pY1234+pY1235.