Product Name: MKK7-pT275
Product Number: AB-PK717
Size: 25 µg      Price:89.00
      $US
Target Full Name: MAPK/ERK protein-serine kinase 7 (MKK7); Dual specificity mitogen-activated protein kinase kinase 7

Target Alias: c-Jun N-terminal kinase kinase 2; JNK activating kinase 2; JNK kinase 2; JNK-activating kinase 2; JNKK2; Jun N-terminal kinase kinase 2; MAP2K7; MKK7; PRKMK7; O14733; ENSG00000076984

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK717

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T275

Protein UniProt: O14733

Protein SigNET: O14733

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MKK7 (MEK7, MAP2K7) sequence peptide Cat. No.: PE-04ANW90

Antibody Immunogen Sequence: KAK(pT)RSA(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues K272 to A278; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Medium immunoreactivity with recombinant human MKK7 on protein dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-52 kDa.

Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEK1 or MKK4.

Related Product 1: MKK7-pT275 blocking peptide

Related Product 2: MKK7 pan-specific antibody (Cat. No.: AB-NK106-5P)

Related Product 3: MKK7-2 pan-specific antibody (Cat. No.: AB-NK106-4)

Related Product 4: MKK7-3 pan-specific antibody (Cat. No.: AB-NK106-5)

Scientific Background: MKK7 (MAP2K7, MEK7) is a dual-specificity protein kinase that functions in the MAP kinase (MAPK) and the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signalling pathways. It acts as an essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signalling pathway. It is activated by phosphorylation at S271 and T275 in the kinase activation loop by specific MAP kinase kinase kinases including MAP3K1 (MEKK1), MAP3K3 (MEKK3), MAP3K11 (MLK3) and MAP3K12 (DLK). With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. MEK7 has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signalling pathway is also involved in mitochondrial death signalling pathway, including the release cytochrome c, leading to apoptosis.
Figure 1. Epitope mapping of MKK7-pT275 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to MKK7-pT275.