Product Name: MSK2-pT687
Product Number: AB-PK725
Size: 25 µg      Price:89.00
      $US
Target Full Name: Ribosomal protein S6 kinase alpha-4

Target Alias: Kinase MSK2; KS6A4; Ribosomal protein kinase B; Ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSK-B; Similar to ribosomal protein S6 kinase, 90kD, polypeptide 4; Similar to ribosomal protein S6 kinase, polypeptide 4; CCDS8073.1; ENSG00000162302

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK725

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T687

Protein UniProt: O75676

Protein SigNET: O75676

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MSK2 (RPS6KA4) sequence peptide Cat. No.: PE-04AKR95

Antibody Immunogen Sequence: PLR(pT)PDC(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues P684 to C690; In the C-terminal part of the kinase after the second catalytic domain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 83-90 kDa.
Antibody Specificity: Very high

Antibody Cross Reactivity: Almost no significant cross-reactivities in A431, Jurkat and T98G cells; in Jurkat cells, phenylarsine oxide (PAO) increases detection of 15 kDa protein.

Related Product 1: MSK2-pT687 blocking peptide

Related Product 2: MSK2-pT194 + pS196 phosphosite-specific antibody (Cat. No.: AB-PK868)

Related Product 3: MskSubtide - MSK1 (RPS6KA5) protein kinase substrate peptide

Scientific Background: MSK2 (RPS6KA4) is a protein-serine/threonine kinase of the AGC group and RSK family. It is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. It phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. It plays an essential role in the control of RELA transcriptional activity in response to TNF. It phosphorylates S10 as well as S28 of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. It mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. It appears to induce activation of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. F709A substitution is associated with strongly elevated basal activity. MSK2 is activated by phosphorylation at S196, S343, S347, T360, T568 and T687. Aberrant activation of members of the family have found to be linked with many human diseases, such as breast and prostate cancers.
Figure 1. Phosphopeptide dot blot epitope mapping with MSK2-pT687 antibody.