Product Name: Nek6-pS206
Product Number: AB-PK734
Size: 25 µg      Price:89.00
      $US
Target Full Name: NIMA (never-in-mitosis)-related protein-serine kinase 6

Target Alias: Kinase Nek6; NIMA; NIMA (never in mitosis gene a)-related expressed kinase 6; NIMA (never in mitosis gene a)-related kinase 6; NIMA-related kinase 6; NIMA-related serine/threonine kinase; Putative serine-threonine protein kinase; SID6-1512

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK734

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S206

Protein UniProt: Q9HC98

Protein SigNET: Q9HC98

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Nek6 sequence peptide Cat. No.: PE-04AOE99

Antibody Immunogen Sequence: AAH(pS)LVG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues A203 to G209; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 36-38 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells.

Related Product 1: Nek6-pS206 blocking peptide

Scientific Background: Nek6 is a protein-serine/threonine kinase that is a member of the Other group of protein kinases in the Nek family. This kinase is highly expressed and widely distributed in most tested human tissues except apparently in the brain and spinal cord. Nek6 is closely related in its catalytic domain to the protein-serine/threonine kinase NimA of Aspergillus nidulans. Nek6 is phosphorylated and activated during M phase of the cell cycle, and its inhibition facilitates apoptosis. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis. Nek6 may be an oncoprotein (OP). Nek6 may contribute to tumourigenesis by suppressing p53/TP53-induced cancer cell senescence. Nek6 inhibition sensitizes human cancer cells towards premature senescence.
Figure 1. Epitope mapping of Nek6-pS206 antibody with similar phosphopeptides on dot blots.


Figure 2. Epitope mapping of Nek6-pS206 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to Nek6-pS206.