Product Name: PDK1-pS241
Product Number: AB-PK760
Size: 25 µg      Price:89.00
      $US
Target Full Name: 3-phosphoinositide-dependent protein-serine kinase 1

Target Alias: 3-phosphoinositide dependent protein kinase-1; HPDK1; Kinase PDPK1; MGC20087; MGC35290; MPDK1; PDPK1; PkB kinase; PkB-like; PkB-like 2; Protein kinase B kinase

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK760

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S241

Protein UniProt: O15530

Protein SigNET: O15530

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PDK1 (PDPK1) sequence peptide Cat. No.: PE-04AKW99

Antibody Immunogen Sequence: RAN(pS)FVG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R238 to G244; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-65 kDa.
Antibody Specificity: High-very high

Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2 and HeLa cells; in HeLa cells, phenylarsine oxide (PAO) increases 25 kDa protein.

Related Product 1: PDK1-pS241 blocking peptide

Related Product 2: PDK1-NT pan-specific antibody (Cat. No.: AB-NK126-4)

Related Product 3: PDKSubtide - PDK1 (PDPK1) protein kinase substrate peptide

Scientific Background: PDK1 (PDPK1) is a protein-serine/threonine kinase of the AGC group and PKB family. It activates, via phosphorylation, many members of the AGC family of protein kinases. It is activated by phosphorylaton at Y9, S160, S241, Y373, and Y376. MELK-mediated phosphorylation can be inhibited through a T354A mutation. PDK1 phosphorylation of MAP3K5 can be ablated through the S394A and S398A mutations. PKB has been observed to be phosphorylated more via a T513E mutation in PDK1. Its phosphotransferase activity can be reduced 3-fold with a A277V mutation. PDK1 is important in mediating migration and metastasis of breast cancer cells.
Figure 1. Epitope mapping of PDK1-pS241 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PDK1-pS241.