Product Name: Plk4-pT170
Product Number: AB-PK780
Size: 25 µg      Price:89.00
      $US
Target Full Name: Polo-like kinase-serine 4 (SAK)

Target Alias: Plk-4; Polo-like kinase 4; Sak; Serine,threonine-protein kinase 18; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; STK18; CCDS3735.1; O00444; ENSG00000142731

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK780

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T170

Protein UniProt: O00444

Protein SigNET: O00444

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Plk4 (SAK; STK18) sequence peptide Cat. No.: PE-04AKZ99

Antibody Immunogen Sequence: KHY(pT)LCG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues K167 to G173; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 / 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.

Related Product 1: Plk4-pT170 blocking peptide

Related Product 2: Plk4-MID pan-specific antibody (Cat. No.: AB-NK291-1)

Scientific Background: Plk4 (SAK) is a protein-serine/threonine kinase of the Other group and Plk family. It has a key function in centriole duplication through the recruitment of the centriole biogenesis proteins SASS6, CENPJ/CPAP, CCP110, CEP135, and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. It phosphorylates S151 of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. It is involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activation of HAND1. It also phosphorylates CDC25C and Chk2 (CHEK2). Its central role in centriole replication indicates that it may have a possible role in tumourigenesis, as centrosome aberrations are frequently observed in tumours.
Figure 1. Epitope mapping of Plk4-pT170 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to Plk4-pT170.