Product Name: PYK2-pY402
Product Number: AB-PK787
Size: 25 µg      Price:89.00
      $US
Target Full Name: Protein tyrosine kinase 2 beta

Target Alias: CADTK; CAK beta; CAKB; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; FADK 2; FADK2, FAK2; FAK2; Related adhesion focal tyrosine kinase; RAFTK

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK787

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y402

Protein UniProt: Q14289

Protein SigNET: Q14289

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PYK2 (PTK2B) sequence peptide Cat. No.: PE-04AGK99

Antibody Immunogen Sequence: SDI(pY)AEI(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues S399 to I405; In the region between the FERM_M domain and the protein kinase catalytic domain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-120 kDa.

Antibody Specificity: Very high

Related Product 1: PYK2-pY402 blocking peptide

Related Product 2: PYK2-pY402 phosphosite-specific antibody (Cat. No.: AB-PK788)

Related Product 3: PYK2-pY579+pY580 phosphosite-specific antibody (Cat. No.: AB-PK789)

Related Product 4: Pyk2Subtide - Pyk2 (PTK2B) protein kinase substrate peptide

Scientific Background: PYK2 (PTK2B, FAK2) is a protein-tyrosine kinase of the TK group and Fak family. It is a non-receptor kinase that has a role in actin cytoskeleton rearrangement, cell migration, cell polarization, adhesion, bone remodeling, and controlling amount of marginal B-cells' migration and presence in the spleen. Phosphorylation at TY402 increases phosphotransferase activity and induces interaction with ErbB2, RasGAP and Src. Phosphorylation at Y579 increases its phosphotransferase activity. NPHP1 interaction with PYK2 has been interrupted with a P859A mutation. A phosphorylation site of PYK2 can be inhibited with an Y881F mutation, which will also impair GRB2 interaction.
Figure 1. Epitope mapping of PYK2-pY402 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PYK2-pY402.