Target Full Name: Serine/threonine-protein kinase RIO1

Target Alias: AD034; BA288G3.1; FLJ30006; Kinase RIOK1; RIO kinase 1

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK794

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y466

Protein UniProt: Q9BRS2

Protein SigNET: Q9BRS2

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human RIOK1 sequence peptide Cat. No.: PE-04APK01

Antibody Immunogen Sequence: NIL(pY)QTV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues N463 to V469; In the C-terminal third of the kinase after the catalytic domain. This is a major in vivo phosphorylation site in RIOK1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.