Product Name: SgK269-pY635
Product Number: AB-PK811
Size: 25 µg      Price:89.00
      $US
Target Full Name: Tyrosine-protein kinase SgK269; Pseudopodium-enriched atypical kinase 1

Target Alias: FLJ21140; FLJ34483; PEAK1; KIAA2002 protein (partial sequence); SG269; Tyrosine-protein kinase SgK269: Sugen kinase 269

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK811

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y635

Protein UniProt: Q9H792

Protein SigNET: Q9H792

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SgK269 (PEAK1) sequence peptide Cat. No.: PE-04AHC99

Antibody Immunogen Sequence: PNA(pY)DNL(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues P632 to L638; In the N-terminal half of the protein. This is the major in vivo phosphorylation site in SgK269.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 190-210 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: Almost no significant cross-reactivities detected in A431 cells.

Related Product 1: SgK269-pY635 blocking peptide

Scientific Background: SgK269 (PEAK1, pseudopodium-enriched atypical kinase 1) is a protein-serine/threonine kinase of the Other group and NKF3 family. It appears to function in the regulation of cell spreading and migration on fibronectin substrates. In addition, SgK269 is predicted to either directly or indirectly affect the phosphorylation of cytoskeleton-associated proteins, such as MAPK1/ERK and PXN. SgK269 has been demonstrated to function downstream of eIF5A1 and eIF5A2, translation initiation factors that have been implicated in several forms of human cancer. For example, the expression of these factors is required for the growth of pancreatic ductal adenocarcinoma (PDAC) cells in vivo and their overexpression in mice models leads to enhanced PDAC cell growth and tumour formation. Downstream activity of PEAK1 is required for the PDAC growth promoting effects of the eIF5A factors, indicating a role for SgK269 in the tumourigenesis of PDAC.
Figure 1. Epitope mapping of SgK269-pY635 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to SgK269-pY635.