Product Name: TBK1-pS172
Product Number: AB-PK828
Size: 25 µg      Price:89.00
      $US
Target Full Name: TANK binding kinase TBK1; Serine/threonine-protein kinase TBK1

Target Alias: FLJ11330; Kinase TBK1; NAK; NF-KB-activating kinase NAK; TANK binding kinase TBK1; TANK-binding kinase 1; CCDS8968.1; ENSG00000183735

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK828

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S172

Protein UniProt: Q9UHD2

Protein SigNET: Q9UHD2

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human TBK1 sequence peptide Cat. No.: PE-04ALG90

Antibody Immunogen Sequence: QFV(pS)LYG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues Q169 to G175; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in HepG2 cellsStrong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-90 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: Almost no significant cross-reactivities detected with HepG2 cells. Strongly cross-reacts with a ~55 kDa protein in sea star oocytes.

Related Product 1: TBK1-pS172 blocking peptide

Related Product 2: TBK1Subtide - TBK1 protein kinase substrate peptide

Scientific Background: TBK1 (NAK) is a protein-serine/threonine kinase of the Other group and IKK family. It plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. To establish such an antiviral state. It mediates the immune response via signalling with TANK, TRAF3, IRF3, IRF7, and DDX3X. It can act as a scaffold for the proteins FADD, AZI2, MAVS, TRADD, TANK or TBKBP1/SINTBAD. TBK1 can be ubiquitinated at K30 and K401. TBK1 is phosphorylated and activated at S172A, Y325, and Y577. It can phosphorylate NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. It phosphorylates and activates AKT1.
Figure 1. Epitope mapping of TBK1-pS172 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to TBK1-pS172.