Product Name: TrkA-pY680+pY681
Product Number: AB-PK837
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Nerve growth factor (NGF) receptor-tyrosine kinase; High affinity nerve growth factor receptor
Target Alias: DKFZp781I14186; MTC; NTrk1; P140-TrkA; Slow nerve growth factor receptor; Trk; Trk1; Trk4;Trk-A; p140-TrkA; Q04227; CCDS1161.1; Q04227; ENSG00000198400
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK837
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y680+Y681
Protein UniProt: P04629
Protein SigNET: P04629
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: DKFZp781I14186; MTC; NTrk1; P140-TrkA; Slow nerve growth factor receptor; Trk; Trk1; Trk4;Trk-A; p140-TrkA; Q04227; CCDS1161.1; Q04227; ENSG00000198400
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK837
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y680+Y681
Protein UniProt: P04629
Protein SigNET: P04629
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TrkA (NGFR; NTrk1) sequence peptide Cat. No.: PE-04AHV95
Antibody Immunogen Sequence: STD(pY)(pY)RVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S677 to G684; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Immunogen Sequence: STD(pY)(pY)RVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S677 to G684; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Related Product 1: TrkA-pY680+pY681 blocking peptide
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Related Product 1: TrkA-pY680+pY681 blocking peptide
Related Product 2: TrkB-pY516 phosphosite-specific antibody (Cat. No.: AB-PK838)
Related Product 3: TrkB-pY702 phosphosite-specific antibody (Cat. No.: AB-PK839)
Related Product 4: TrkC-pY709+pY710 phosphosite-specific antibody (Cat. No.: AB-PK840)
Related Product 5: TrkSubtide - TrkA (NTRK1) protein kinase substrate peptide
Related Product 3: TrkB-pY702 phosphosite-specific antibody (Cat. No.: AB-PK839)
Related Product 4: TrkC-pY709+pY710 phosphosite-specific antibody (Cat. No.: AB-PK840)
Related Product 5: TrkSubtide - TrkA (NTRK1) protein kinase substrate peptide
Scientific Background: TrkA (NTrk1) is a protein-tyrosine kinase of the TK group and Trk family. It is a receptor protein-tyrosine kinase with high affinity for nerve growth factor (NGF). The TrkA protein functions in the development and maturation of both the central nervous system (CNS) and peripheral nervous system (PNS) through the regulation of neuronal proliferation, differentiation, and survival, specifically of sympathetic neurons. Upon binding to NGF, TrkA undergoes homodimerization and activating autophosphorylation. Autophosphorylation of Y496 increases phosphotransferase activity and induces interaction with FRS2 and Shc1. Autophosphorylation of Y757 increases phosphotransferase activity. Autophosphorylation of Y791 increases phosphotransferase activity and induces interaction with PLCg1. Phosphorylation of Y701 inhibits phosphotransferase activity and induces interaction with CLTC and LAMP1. These interactions mediate NGF effects on cell survival and proliferation. TrkA appears to be an oncoprotein (OP). Overactive forms of the TrkA protein are known to have oncogenic properties and promote tumourigenesis. Chromosomal translocations involving the TrkA gene have been observed in thyroid papillary carcinoma tissue samples.
Figure 1. Epitope mapping of TrkA-pY680+pY681 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to TrkA-pY680+pY681.
© Kinexus Bioinformatics Corporation 2017