Target Full Name: Alpha-actinin-1

Target Alias: actinin 1 smooth muscle; actinin, alpha 1; ACTN1; alpha-actinin 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1

Product Type Specific: Alpha-actinin phosphosite-specific antibody

Antibody Code: PN502

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y246

Protein UniProt: P12814

Protein SigNET: P12814

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human ACTN1 sequence peptide Cat. No.: PE-04AIC99

Antibody Immunogen Sequence: SSF(pY)HAF(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues S243 to F249; In the second CH domain. This is the major in vivo phosphorylation site in ACTN1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.