Product Name: PECAM-1-pY713
Product Number: AB-PN523
Size: 25 µg      Price:89.00
      $US
Target Full Name: Platelet endothelial cell adhesion molecule

Target Alias: Adhesion molecule; CD31; CD31 antigen; CD31/EndoCAM; EndoCAM; FLJ58394; GPIIA'; PECA1; PECAM-1; PECAM-1, CD31/EndoCAM; PECAM1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule

Product Type Specific: PECAM-1 phosphosite-specific antibody

Antibody Code: PN523

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y713

Protein UniProt: P16284

Protein SigNET: P16284

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PECAM-1 sequence peptide Cat. No.: PE-04AEZ99

Antibody Immunogen Sequence: ETV(pY)SEV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E710 to V716; In the C-terminal region of the protein after the transmembrane domain. This is the major in vivo phosphorylation site in PECAM-1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 125-135 kDa.

Antibody Specificity: Medium-High

Antibody Cross Reactivity: In Jurkat cells, phenylarsine oxide (PAO) increases detection of 75-85 kDa and 15 kDa cross-reactive proteins.

Related Product 1: PECAM-1-pY713 blocking peptide

Scientific Background: PECAM1 is a cell adhesion protein that is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Phosphorylation at Y690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. It prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, to promote tethering of dying cells to phagocytes. During apoptosis, the inside-out signalling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, and triggers the process of engulfment. Isoform Delta15 is unable to protect against apoptosis. It modulates BDKRB2 activation. It regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Figure 1. Epitope mapping of PECAM-1-pY713 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PECAM-1-pY713.