Product Name: PGK1-pY196
Product Number: AB-PN525
Size: 25 µg      Price:89.00
      $US
Target Full Name: Phosphoglycerate kinase 1

Target Alias: Cell migration-inducing gene 10 protein; MGC117307; MGC142128; MGC8947; MIG10; PGK1; PGKA; Primer recognition protein 2; PRP 2

Product Type Specific: PGK1 phosphosite-specific antibody

Antibody Code: PN525

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y196

Protein UniProt: P00558

Protein SigNET: P00558

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PGK1 sequence peptide Cat. No.: PE-04ADF85

Antibody Immunogen Sequence: ELN(pY)FAK(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E193 to K199; In the middle of the PGK domain. This is the major in vivo phosphorylation site in PGK1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 44-48 kDa.
Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and T98G cells.

Related Product 1: PGK1-pY196 blocking peptide

Related Product 2: PKG1Subtide - PKG1 (PRKG1) protein kinase substrate peptide

Scientific Background: PGK1 is the second glycolytic enzyme in the pathway to produce pyruvate from glucose, and also acts as a polymerase alpha cofactor protein (primer recognition protein). It has also been implicated to play a role in sperm motility. It catalyzes the reaction: ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Figure 1. Epitope mapping of PGK1-pY196 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PGK1-pY196.