Product Name: PIK3R1-pY580
Product Number: AB-PN527
Size: 25 µg      Price:89.00
      $US
Target Full Name: Phosphatidylinositol 3-kinase regulatory subunit alpha

Target Alias: GRB1; P85; P85A; P85-ALPHA; Phosphatidylinositol 3-kinase regulatory alpha subunit; Phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3K; PI3K p85-alpha; PI3-kinase p85-alpha; PtdIns-3-kinase p85-alpha

Product Type Specific: Lipid kinase phosphosite-specific antibody

Antibody Code: PN527

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y580

Protein UniProt: P27986

Protein SigNET: P27986

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PIK3R1 sequence peptide Cat. No.: PE-04AEX85

Antibody Immunogen Sequence: RDQ(pY)LMW(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R577 to W583; In the region between the two SH2 domains in the C-terminus half of the protein. This is one of the major in vivo phosphorylation sites inPIK3R1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.

Related Product 1: PIK3R1-pY580 blocking peptide

Related Product 2: PIK3R1-pY467 phosphosite-specific antibody (Cat. No.: AB-PN526)

Scientific Background: PIK3R1 binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. It also mediates cellular signalling in response to various growth factors that bind to other receptor-tyrosine kinases, including EGF, IGF1, VEGFA and PDGF, as well as downstream proteins such as insulin-receptor substrate (IRS) proteins. It plays a role in ITGB2 signalling (PubMed:17626883, PubMed:19805105, PubMed:7518429), and modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver to improve glucose tolerance (PubMed:20348923).
Figure 1. Epitope mapping of PIK3R1-pY580 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PIK3R1-pY580.