Target Full Name: Protein phosphatase 1 regulatory subunit 11

Target Alias: HCG V; HCG-V; HCGV; Hemochromatosis candidate gene V protein; inhibitor-3; IPP3; MGC125741; MGC125742; MGC125743; PP1RB; PPP1R11; Protein phosphatase 1 regulatory subunit 11; protein phosphatase 1, regulatory (inhibitor) subunit 11; protein phosphatase 1, regulatory (inhibitor) subunit 11, isoform 1; Protein phosphatase inhibitor 3; t-complex-associated-testis-expressed 5; TCTE5; TCTEX5

Product Type Specific: PPP1R11 (HCG V) phosphosite-specific antibody

Antibody Code: PN532

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y64

Protein UniProt: O60927

Protein SigNET: O60927

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PPP1R11 (HCG V) sequence peptide Cat. No.: PE-04ADI99

Antibody Immunogen Sequence: CCI(pY)EKP(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues C61 to P67; In the PPI_Ypi1 domain. This is the major in vivo phosphorylation site in PPP1R11.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.