Product Name: STAT3-pY705+pT708
Product Number: AB-PN539
Size: 25 µg      Price:89.00
      $US
Target Full Name: Signal transducer and activator of transcription 3

Target Alias: Acute-phase response factor; APRF

Product Type Specific: STAT3 phosphosite-specific antibody

Antibody Code: PN539

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y705+T708

Protein UniProt: P40763

Protein SigNET: P40763

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human STAT3 sequence peptide Cat. No.: PE-04ADO95

Antibody Immunogen Sequence: AAP(pY)LK(pT)(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues A702 to T708; In the C-terminus of the transcription factor. Y705 is the major in vivo phosphorylation site in STAT3.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-88 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2 and T98G cells.

Related Product 1: STAT3-pY705+pT708 blocking peptide

Scientific Background: STAT3 is a Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, it recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). It mediates cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. It also binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes.
Figure 1. Epitope mapping of STAT3-pY705+pT708 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to STAT3-pY705+pT708.