Product Name: TLN1-pY70
Product Number: AB-PN542
Size: 25 µg      Price:89.00
      $US
Target Full Name: Talin 1

Target Alias: OTTMUSP00000007396; Talin-1; Tln; Tln1

Product Type Specific: TLN1 phosphosite-specific antibody

Antibody Code: PN542

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y70

Protein UniProt: Q9Y490

Protein SigNET: Q9Y490

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human TLN1 sequence peptide Cat. No.: PE-04ADP85

Antibody Immunogen Sequence: ALD(pY)YML(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues A67 to L73; In the N-terminus of the protein. This is the major in vivo phosphorylation site in TLN1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 260-280 kDa.

Related Product 1: TLN1-pY70 blocking peptide

Scientific Background: Talin-1 is a cytoskeletal protein that is found in areas of cell-substratum and cell-cell contacts. It plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein interacts with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein facilitates localization to cell-extracellular matrix junction, whereas the C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
Figure 1. Epitope mapping of TLN1-pY70 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to TLN1-pY70.