Product Name: Vav1-pY826
Product Number: AB-PN543
Size: 25 µg      Price:89.00
      $US
Target Full Name: Vav 1 guanine nucleotide exchange factor

Target Alias: Vav; vav 1 guanine nucleotide exchange factor; Vav proto-oncogene

Product Type Specific: Vav1 phosphosite-specific antibody

Antibody Code: PN543

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y826

Protein UniProt: P15498

Protein SigNET: P15498

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Vav1 sequence peptide Cat. No.: PE-04AEO99

Antibody Immunogen Sequence: GEI(pY)GRV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G823 to V829; In the SH3_1 domain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa cells and sea star oocytes. ~45-47 kDa and ~80 kDa cross-reactive proteins were also detectable in Jurkat cells.

Related Product 1: Vav1-pY826 blocking peptide

Scientific Background: Vav1 is a proto-oncogenic guanine nucleotide exchange factors (GEF) of the Dbl family that couples receptor-protein-tyrosine kinase signals with the activation of the Rho/Rac GTPases to lead to cell differentiation and/or proliferation. It is expressed exclusively in hematopoietic cells and is important in hematopoiesis, by playing roles in T-cell and B-cell development and activation.
Figure 1. Epitope mapping of VAV1-pY826 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to VAV1-pY826.