Target Full Name: Protein arginine N-methyltransferase 5

Target Alias: 72 kDa ICln-binding protein; ANM5; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; HRMT1L5; IBP72; Jak-binding protein 1; JBP1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; SKB1Hs

Product Type Specific: PRMT5 phosphosite-specific antibody

Antibody Code: PN549

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T634

Protein UniProt: O14744

Protein SigNET: O14744

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PRMT5 sequence peptide Cat. No.: PE-04ARI90

Antibody Immunogen Sequence: GRSY(pT)IGL(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G630 to L637; At the C-terminus of the protein.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.