Product Name: CREB-pS133
Product Number: AB-PN553
Size: 25 µg      Price:89.00
      $US
Target Full Name: cAMP-response element-binding protein 1

Target Alias: CAMP responsive element binding protein 1; CAMP-response element binding protein; CREB1; CREB-1

Product Type Specific: CREB1 phosphosite-specific antibody

Antibody Code: PN553

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S133

Protein UniProt: P16220

Protein SigNET: P16220

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CREB1 sequence peptide Cat. No.: PE-04ARE90
Antibody Immunogen Sequence: RRP(pS)YRK(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R130 to K136; In the PKID domain.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431, HeLa and MCF7 cells.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-47 kDa.
Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa and MCF7 cells.

Related Product 1: CREB-pS133 blocking peptide

Scientific Background: CREB1 is a member of the cAMP responsive element binding protein family of transcription factors that are critical mediators of gene expression in response to extracellular signals and are essential regulators of adaptive behavior and long-term memory formation. Activation of CREB1 by protein kinase A-mediated phosphorylation has been implicated in the survival of mammalian cells. Mice lacking CREB in the central nervous system during development show extensive apoptosis of postmitotic neurons. CREB binds as homo- and heterodimers to promoters containing CRE and activator protein 1 (AP-1) sites to alter target-gene expression.
Figure 1. Epitope mapping of CREB-pS133 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to CREB-pS133.