Product Name: RPS6-pS235+pS236+pS240
Product Number: AB-PN559
Size: 25 µg      Price:89.00
      $US
Target Full Name: 40S Ribosomal S6

Target Alias: Phosphoprotein NP33; Ribosomal protein S6; RS6; S6 Ribosomal Protein

Product Type Specific: Ribosomal proteinS6 phosphosite-specific antibody

Antibody Code: PN559

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S235+S236+S240

Protein UniProt: P62753

Protein SigNET: P62753

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human RPS6 sequence peptide Cat. No.: PE-04AQR95

Antibody Immunogen Sequence: RRL(pS)(pS)LRA(pS)TS(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R232 to S242; In the C-terminus of the ribosomal protein. These are the major in vivo phosphorylation sites in the S6 protein.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in MCF7 + insulin (homogenized in SDS-PAGE buffer).

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 28-34 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.

Related Product 1: RPS6-pS235+pS236+pS240 blocking peptide

Related Product 2: RPS6 (230-238) KinSub - 40S ribosomal protein S6 (K230-R238, human) peptide; p70S6K protein kinase substrate peptide

Related Product 3: RPS6 (230-239) KinSub - 40S ribosomal protein S6 (K230-A239, human) peptide; RSK protein kinase substrate peptide
Scientific Background: RPS6 plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA that are important in protein synthesis. It belongs to the ribosomal protein S6e family. Growth factor stimulation of cultured cells leads to stong phosphorylation of RPS6 at multiple sites located in its C-terminus, which enhances it translational activity. This phosphorylation may be mediated by several protein kinases including those in the p70 S6 kinase and RSK families.
Figure 1. Epitope mapping of RPS6-pS235+pS236+pS240 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to RPS6-pS235+pS236+pS240.