Product Name: RPS6-pS235+pS236+pS240
Product Number: AB-PN559
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: 40S Ribosomal S6
Target Alias: Phosphoprotein NP33; Ribosomal protein S6; RS6; S6 Ribosomal Protein
Product Type Specific: Ribosomal proteinS6 phosphosite-specific antibody
Antibody Code: PN559
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S235+S236+S240
Protein UniProt: P62753 Protein SigNET: P62753 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human RPS6 sequence peptide Cat. No.: PE-04AQR95
Antibody Immunogen Sequence: RRL(pS)(pS)LRA(pS)TS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R232 to S242; In the C-terminus of the ribosomal protein. These are the major in vivo phosphorylation sites in the S6 protein.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in MCF7 + insulin (homogenized in SDS-PAGE buffer).
Antibody Species Reactivity: Human
Scientific Background: RPS6 plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA that are important in protein synthesis. It belongs to the ribosomal protein S6e family. Growth factor stimulation of cultured cells leads to stong phosphorylation of RPS6 at multiple sites located in its C-terminus, which enhances it translational activity. This phosphorylation may be mediated by several protein kinases including those in the p70 S6 kinase and RSK families.
Figure 1. Epitope mapping of RPS6-pS235+pS236+pS240 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to RPS6-pS235+pS236+pS240.