Target Full Name: CTP: phosphocholine cytidylyltransferase isoform A

Target Alias: CCT A; CCT-alpha; CCTA; Choline-phosphate cytidylyltransferase A; CT; CT A; CTA; CTP:phosphocholine cytidylyltransferase A; CTPCT; PCY1A; PCYT1; PCYT1A; phosphate cytidylyltransferase 1, choline, alpha; Phosphorylcholine transferase A

Product Type Specific: PCYT1A phosphosite-specific antibody

Antibody Code: PN561

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S329+S331

Protein UniProt: P49585

Protein SigNET: P49585

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PCYT1A; CTPCT; CCTA sequence peptide

Antibody Immunogen Sequence: TRER(pS)P(pS)PSF(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues T325 to F334; Near the C-terminus of the protein. These are among the main in vivo phosphorylation sites in PCYT1A.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.