Full Name: Human Embryonic Kidney cells - 293-Cytosolic lysate

Product Type Specific: Cytosolic lysate in SDS-PAGE sample buffer

Product Description: Cytosolic lysate boiled in SDS-PAGE sample buffer prepared from HEK293 cells that were cultivated to 90% confluency and deprived of serum for 18 hours prior to harvesting

Product Method: HEK293 cells were cultivated to 90% confluency and deprived of serum for 18 hours prior to harvesting. Lysates were prepared from scrapped cells that were homogenized by sonication in buffer formulated with 60 mM ß-glycerophosphate, pH 7.2, 20 mM MOPS, 20 mM sodium pyrophosphate, 30 mM sodium fluoride, 5 mM EDTA, 3 mM benzamidine, 2 mM EGTA, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonylfluoride, 1 mM dithiothreitol, 10 µM leupeptin, and 5 µM pepstatin A. Cytosolic lysates were prepared following sonication and 30 min ultracentrifugation at 100,000 rpm. Lysates were further diluted in SDS-PAGE sample buffer at a final concentration of 2 mg/ml.

Storage Conditions: -70°C

Lysate Use Description: For testing antibodies by immunoblotting.

Related Product 1: Cat. No.: LC201F - HEK293 cytosolic lysate from untreated cells (3 mg/ml)