Product Name: AMPK/SIK KinSub
Product Number: PE-01ACL95
Size: 200 µg      Price:48.00
1 mg      $US96.00
Peptide Name: AMPK/SIK KinSub

Product Use: For assaying the phosphotransferase activities of 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK1a, UniProt ID Q13131), 5'-AMP-activated protein kinase, catalytic subunit alpha-2 (AMPK1a2, UniProt ID P54646) and Serine-threonine-protein kinase SNF1-like kinase 1 (SIK, UniProt I P57059).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Based on overlapping concensus kinase recognition sequences for SIK and AMPK

Peptide Sequence: AMARAASAAALARRR

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Free carboxyl

Peptide Molecular Mass Calculated: 1542.8 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: SIK; AMPK (A1/B1/G1); (A1/B1/G2); (A1/B1/G3); (A1/B2/G1); (A2/B1/G1)

Scientific Background: AMPKa1 (PRKAA1) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP results in allosteric activation, inducing phosphorylation on T183 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signalling pathways in response to adiponectin, leptin and CAMKK-beta. Phosphorylation at T174 by CAMKK1 and CAMKK2 increases phosphotransferase activity, triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.
References

[1] Hardie DG. The AMP-activated protein kinase pathway--new players upstream and downstream. J Cell Sci. 2004 Nov 1;117(Pt 23):5479-87. PMID: 15509864.

[2] Kahn BB, Alquier T, Carling D, Hardie DG. AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab. 2005 Jan;1(1):15-25. PMID: 16054041.