Product Name: GRKtide KinSub
Product Number: PE-01ACS95
| Size: | 200 µg | Price: | 36.00 | |
| 1 mg | $US | 73.00 | ||
| 5 mg | 172.00 |
Peptide Name: GRKtide KinSub
Product Use: For assaying the phosphotransferase activities of Beta-adrenergic receptor kinase 1 (UniProt ID P25098) as well as GRK2 (UniProt ID P25098), GRK3 (UniProt ID P35626), GRK4 (UniProt ID P32298), GRK5 (UniProt ID P34947), GRK6 (UniProt ID P43250), GRK7 (UniProt ID Q8WTQ7) and possibly Casein kinase I, alpha isoform (CK1a, UniProt ID P48729).
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Based on common specificity of GRK's
Peptide Sequence: CRRREEEEESAAA
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Free carboxyl
Product Use: For assaying the phosphotransferase activities of Beta-adrenergic receptor kinase 1 (UniProt ID P25098) as well as GRK2 (UniProt ID P25098), GRK3 (UniProt ID P35626), GRK4 (UniProt ID P32298), GRK5 (UniProt ID P34947), GRK6 (UniProt ID P43250), GRK7 (UniProt ID Q8WTQ7) and possibly Casein kinase I, alpha isoform (CK1a, UniProt ID P48729).
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Based on common specificity of GRK's
Peptide Sequence: CRRREEEEESAAA
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Free carboxyl
Peptide Molecular Mass Calculated: 1536.2 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: GRK1; GRK2; GRK5; GRK6; GRK7
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: GRK1; GRK2; GRK5; GRK6; GRK7
Scientific Background: GRK2 (also known as beta-adrenergic receptor kinase 1) is a member of the AGC group of protein kinases in the GRK family, and BARK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of GRK2 are highly conserved in vertebrates, including amphibians. It is activated by binding a Gbeta/gamma subunit complex. Phosphorylation of S29 increases its phosphotransferase activity. It specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. It is a key regulator of LPAR1 signalling, by competing with RALA for binding to LPAR1. It desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
© Kinexus Bioinformatics Corporation 2017