Product Name: AcCoACarbox (73-85) S77A, KinSub
Product Number: PE-01ADR95
Size: 200 µg      Price:48.00
1 mg      $US96.00
5 mg      210.00
Peptide Name: AcCoACarbox (73-85) S77A, KinSub

Product Use: For assaying the phosphotransferase activities of 5'-AMP-activated protein kinase catalytic subunit alpha-1 (UniProt ID Q13131) and 5'-AMP-activated protein kinase, catalytic subunit alpha-2 (AMPK1a2, UniProt ID P54646).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Based on Acetyl-Coenzyme A carboxylase phosphosite

Peptide Sequence: HMRSAMSGLHLVKRR

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Free carboxyl

Peptide Molecular Mass Calculated: 1336.6 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: AMPK (A1/B1/G1); (A1/B1/G2); (A1/B1/G3); (A1/B2/G1); (A2/B1/G1)

Scientific Background: AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signalling pathways in response to adiponectin, leptin and CAMKK-beta.