Product Name: KinSub1DDLYF
Product Number: PE-01AGP95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1DDLYF
Product Use: For assaying the phosphotransferase activity of NT-3 growth factor receptor (TrkC, UniProt ID Q16288). The KinSub1DDLYF peptide demonstrated very high phosphotransferase activity with Brk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDDLYFGPGGGG
Product Use: For assaying the phosphotransferase activity of NT-3 growth factor receptor (TrkC, UniProt ID Q16288). The KinSub1DDLYF peptide demonstrated very high phosphotransferase activity with Brk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDDLYFGPGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1353 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1353 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Scientific Background: TrkC is one of several protein kinases that can phosphorylate KinSub1DDLYF. Human TrkC is a receptor protein-tyrosine kinase of 839 amino acid length, with a predicted molecular mass of 94,428 Da. It is a member of the TK group of protein kinases in the Trk family. This kinase shows high variability in human tissue distribution with the highest levels detected in brain, liver, spinal cord and thymus. Orthologues of TrkC are highly conserved in mammals and birds. It is the high affinity catalytic receptor for the neurotrophin NT-3. TrkC is activated by phosphorylation at Y516. Phosphorylation of Y834 of TrkC induces interaction with PLCg1 and Shc1. TRKC mediates the multiple cellular effects of the NT-3 neurotrophic factor, which includes neuronal differentiation and survival (1). TrkC has been implicated in insulin signalling pathway through interactions with the MUSK protein receptor and the VEGF receptor. Mutations in the TrkC gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers (2). TrkC has also been linked with the development of pediatric mesenchymal, colon, and large lung cell carcinomas (LLCC), and gastric and lung adenocarcinomas.
References
[1] Lamballe F, Klein R, Barbacid M. trkC, a new member of the trk family of tyrosine protein kinases, is a receptor for neurotrophin-3. Cell. 1991 Sep 6;66(5):967-79. PMID:1653651
[2] Marchetti A, Felicioni L, Pelosi G, Del Grammastro M, Fumagalli C, Sciarrotta M, Malatesta S, Chella A, Barassi F, Mucilli F, Camplese P, D'Antuono T, Sacco R, Buttitta F. Frequent mutations in the neurotrophic tyrosine receptor kinase gene family in large cell neuroendocrine carcinoma of the lung. Hum Mutat. 2008 May;29(5):609-16. PMID: 18293376
[1] Lamballe F, Klein R, Barbacid M. trkC, a new member of the trk family of tyrosine protein kinases, is a receptor for neurotrophin-3. Cell. 1991 Sep 6;66(5):967-79. PMID:1653651
[2] Marchetti A, Felicioni L, Pelosi G, Del Grammastro M, Fumagalli C, Sciarrotta M, Malatesta S, Chella A, Barassi F, Mucilli F, Camplese P, D'Antuono T, Sacco R, Buttitta F. Frequent mutations in the neurotrophic tyrosine receptor kinase gene family in large cell neuroendocrine carcinoma of the lung. Hum Mutat. 2008 May;29(5):609-16. PMID: 18293376
© Kinexus Bioinformatics Corporation 2017